We provide two methods, Taqman assays and Sybr Green based realtime RT-PCR, to help determine the gene transcription quantitatively or semi-quantitatively.
- Sample preparation
Cells or tissues should be as fresh as possible and stored at -80°C or in liquid nitrogen.
- RNA extraction and reverse transcription
To ensure the RNA quality we prefer extracting mRNA or total RNA in our laboratory. All RNA samples are reversely transcribed to cDNA immediately after the extraction and stored at -80°C.
- Quantitative and semi-quantitative realtime PCR
We have various house keeping gene standards for qPCR and semi-qPCR . Please contact us at ncgenemaster@gmail.com for more detail.
